Label-free quantitation, an extension to 2DB
نویسندگان
چکیده
منابع مشابه
Applying Label-Free Quantitation to Top Down Proteomics
With the prospect of resolving whole protein molecules into their myriad proteoforms on a proteomic scale, the question of their quantitative analysis in discovery mode comes to the fore. Here, we demonstrate a robust pipeline for the identification and stringent scoring of abundance changes of whole protein forms <30 kDa in a complex system. The input is ~100-400 μg of total protein for each b...
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In this study, we present a fully automated tool, called IDEAL-Q, for label-free quantitation analysis. It accepts raw data in the standard mzXML format as well as search results from major search engines, including Mascot, SEQUEST, and X!Tandem, as input data. To quantify as many identified peptides as possible, IDEAL-Q uses an efficient algorithm to predict the elution time of a peptide unide...
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ژورنال
عنوان ژورنال: Amino Acids
سال: 2009
ISSN: 0939-4451,1438-2199
DOI: 10.1007/s00726-009-0317-9